Highland Reel© Photo Healy Racing
Highland Reel attempts a rare double when he bids to follow-up his Coronation Cup success in the Prince of Wales's Stakes at Royal Ascot on Wednesday.
O'Brien's five-year-old won the Secretariat Stakes over a mile and quarter in 2015 and took the King George VI And Queen Elizabeth II Stakes over 12 furlongs at Ascot last summer.
He will be tackling 10 furlongs again this time with O'Brien also fielding Johannes Vermeer in the nine-runner field.
French interests rest on the Jean-Claude Rouget-trained Mekhtaal, winner of the Prix d'Ispahan.
The home team is led by Jack Hobbs from John Gosden's stable.
The five-year-old has had little racing since his Classic campaign when he lifted the Irish Derby, but showed he was as good as ever when winning the Dubai Sheema Classic at Meydan in March.
Godolphin, who are major shareholders in Jack Hobbs, also have Scottish from Charlie Appleby's stable. He has not raced since finishing second in the Caulfield Cup in Australia last October.
Sir Michael Stoute is double-handed with Ulysses and Queen's Trust, while Roger Charlton has Decorated Knight, who took the Tattersalls Gold Cup at the Curragh last month.
The list being completed by Roger Varian's filly Nezwaah.
